Pectinase digestion method was employed for the enhancement of activity of barley leaf.
Ethanol fractionation method was employed for the rapid isolation of immunolological active polysaccharide of barley leaf.
BLE-P-I purified from immunoactive crude polysaccharide showed the potent macrophage stimulating activity.
Glucuronoarabinoxylan and rhamnogalacturonan-I branched mainly with a type II arabinogalactan side chain may be important for expression of the activity in barley leaf.