GdnHCl-induced unfolding intermediate in the mitochondrial carbonic anhydrase VA
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- 作者：Danish Idrees ; Amresh Prakash ; Md. Anzarul Haque ; Asimul Islam ; Md. Imtaiyaz Hassan ; mihassan@jmi.ac.in" class="auth_mail" title="E-mail the corresponding author ; imtiyaz.hassan@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Faizan Ahmad
- 关键词：Carbonic anhydrase VA ; GdnHCl-induced denaturation ; Protein folding ; Molecular dynamics simulation ; 1-Anilinonaphthalene-8-sulfonic acid
- 刊名：International Journal of Biological Macromolecules
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：91
- 期：Complete
- 页码：1151-1160
- 全文大小：2112 K
文摘
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We determined mechanism of GdnHCl-induced unfolding of CAVA.
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GdnHCl-induced unfolding of CAVA is a biphasic and reversible process.
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A pre-molten globule state is observed in GdnHCl-induced unfolding of CAVA.
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The intermediate state is characterized by ANS-binding and near-UV CD.