Disruption of KEX1 gene reduces the proteolytic degradation of secreted two-chain Insulin glargine in Pichia pastoris
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- 作者：Suma Sreenivasa ; suma.sreenivas@biocon.com" class="auth_mail" title="E-mail the corresponding author ; Sateesh M. Krishnaiahb ; Anil H. Shyam Mohana ; Niveditha Mallikarjuna ; Nagaraja Govindappaa ; Amarnath Chatterjeea ; Kedarnath N. Sastrya
- 关键词：Pichia pastoris ; Two-chain glargine ; Cre/lox mechanism ; Gene inactivation ; Marker rescue
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：118
- 期：Complete
- 页码：1-9
- 全文大小：1149 K
文摘
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Kex1 gene inactivation to reduce proteolysis of two-chain glargine in Pichia pastoris.
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Process improvement by reducing product related impurities by gene inactivation.
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Cre/lox mechanism for targeted gene inactivation and marker recycling in Pichia.