Expression of 25 high egg production related transcripts that identified from hypothalamus and pituitary gland in red-feather Taiwan country chickens

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• 作者：Lih-Ren Chen ; Ching-Hsien Chao ; Chih-Feng Chen ; Yen-Pai Lee ; Yi-Ling Chen ; Yow-Ling Shiue
• 关键词：Chicken ; High egg production ; Hypothalamus ; Pituitary gland ; Transcripts ; Quantitative RT-PCR
• 刊名：Animal Reproduction Science
• 出版年：2007
• 出版时间：July 2007
• 年：2007
• 卷：100
• 期：1-2
• 页码：172-185
• 全文大小：210 K

文摘

Expression levels of 33 high egg production candidate transcripts in Red-feather Taiwan country chickens (TCCs) were examined by quantitative reverse-transcription (RT) polymerase chain reactions (PCR) in this study. Candidate transcripts were previously identified from a L2-B (L2-subtract-B) hypothalamus/pituitary gland subtractive cDNA library. In this subtractive cDNA library, two divergently selected strains of TCCs, B and L2 were used. These two strains were originated from one single population and were further subjected (since 1982) to the selections of body weight/comb size (B) and eggs to 40 wk of age (L2), respectively. Hypothalamuses and pituitary glands that sampled from Red-feather TCCs were previously grouped into high (Red-high; n = 20) and low (Red-low; n = 20) egg productions based on the rate of lay after 1st egg (hen-day laying rate; % ). Rates of lay after 1st egg (mean ± S.E.) in the Red-high and the Red-low subpopulations were 72.2 ± 0.6 and 23.0 ± 3.5, respectively (P < 0.01). Quantitative RT-PCR validated that 25 candidate transcripts were significantly higher expressed in the Red-high than in the Red-low hens. These transcripts were ANP32A, BDH, CDC42, CNTN1, COMT, CPE, CTNNB1, DIO2, EIF4E, GARNL1, HSPCA, LAPTM4B, MBP, NAP1L4, NCAM1, PARK7, PCDHA@, PGDS, PLAG1, PRL, RAD21, SAR1A, SCG2, STMN1 and UFM1. Among these transcripts, 15 (79.0 % ), 13 (68.4 % ), and 12 (63.2 % ) genes were annotated to involve in cellular physiological process (GO:0050875), metabolism (GO:0008152) and cell communication (GO:0007154). Identified transcripts that related to high egg production are most active in focal adhesion, adherens junction, MAPK signaling, tight junction and cell adhesion pathways.