Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa

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• 作者：Selim Alcay^a ; ^{salcay@uludag.edu.tr" class="auth_mail" title="E-mail the corresponding author} ; Elif Gokce^a ; M. Berk Toker^a ; N. Tekin Onder^a ; Burcu Ustuner^a ; Ender Uzabacı^b ; Zulfiye Gul^c ; Seda Cavus^a
• 关键词：Freeze-drying ; Goat semen ; Cryopreservation ; Antioxidants ; Incubation resilience
• 刊名：Cryobiology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：72
• 期：3
• 页码：269-273
• 全文大小：587 K

文摘

The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine.