First LCA study of second generation ethanol based on DA and SE pretreatment technologies in India. Enzyme production is identified as the major GHG emissions and energy consumption process in ethanol production. SE is better than DA in terms of ethanol yield and GHG emissions reduction. Surplus electricity produced in the ethanol plant replaces coal based grid electricity and makes the process more greener. Cellulosic ethanol production in India is sustainable from GHG and energy savings perspective.