Diacylglycerol kinase ζ generates dipalmitoyl-phosphatidic acid species during neuroblastoma cell differentiation
详细信息 查看全文
- 作者：Satoru Mizunoa ; Sayaka Kadob ; Kaoru Gotoc ; Daisuke Takahashia ; Fumio Sakanea ; sakane@faculty.chiba-u.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：DG ; diacylglycerol ; DGK ; diacylglycerol kinase ; FBS ; fetal bovine serum ; FIPI ; 5-fluoro-2-indolyl deschlorohalopemide ; I.S. ; internal standard ; LC ; liquid chromatography ; MS ; mass spectrometry ; PA ; phosphatidic acid ; PC ; phosphatidylcholine ; PLD ; phospholipase D ; RA ; retinoic acid
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：8
- 期：Complete
- 页码：352-359
- 全文大小：1782 K
文摘
- 5">
- •
5">We analyzed phosphatidic acid (PA) molecular species in neuroblastoma cells.
- •
We identified a marked increase of 32:0-PA during neuroblastoma cell differentiation.
- •
5">32:0-PA species contained two palmitic acids (16:0 s).
- •
20">Diacylglycerol kinase (DGK) ζ-siRNA decreased 16:0/16:0-PA production.
- •
25">Neurite outgrowth was markedly attenuated by deficiency of DGKζ.