Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest

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• 作者：Ayako Katagi^a ; Li Sui^a ; Kazuyo Kamitori^a ; Toshisada Suzuki^b ; Takeshi Katayama^b ; Akram Hossain^a ; Chisato Noguchi^a ; Youyi Dong^a ; Fuminori Yamaguchi^a ; Masaaki Tokuda^a ; ^{tokuda@med.kagawa-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Health sciences ; Pharmaceutical science ; Plant biology ; Cancer treatment ; Cancer
• 刊名：Heliyon
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：2
• 期：1
• 全文大小：1138 K

文摘

Although various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral, analgesic, and/or antidotal efficacies, we are the first to investigate the role of anti-carcinogenicity of isoamericanol A (IAA) from the seed extract. Our results showed that IAA is capable of inhibiting cell proliferation in a dose-dependent manner on the human cancer cell lines of MCF-7, MDA-MB231, HuH-7, and HeLa. Flow cytometry analysis showed IAA significantly induces cell cycle arrest at G2/M on MCF-7 cells. At both protein and mRNA levels examined by western blot and real-time PCR, the results revealed increased expression of BTG2 (B-cell translocation gene 2), p21 (p21^WAF1/CIPI), and GADD45A (growth arrest and DNA-damage-inducible, alpha) after IAA treatment, but inversed expression in CDK1 (cyclin-dependent kinase 1) and cyclins B1 and B2. All these effects contribute to G2/M cell cycle arrest. Furthermore, these results coincide with the changes in molecular expressions determined by DNA-microarray analysis. Our findings indicate that IAA has an inhibitory effect on cell proliferation of MCF-7 through cell cycle arrest, giving it great potential as a future therapeutic reagent for cancers.