Improvement of efficient in vitro regeneration potential of mature callus induced from Malaysian upland rice seed (Oryza sativa cv. Panderas)

详细信息    查看全文

• 作者：Abd Rahman Jabir Mohd Din^a ; ^{jabir@ibd.utm.my" class="auth_mail" title="E-mail the corresponding author} ; Fauziah Iliyas Ahmad^b ; Alina Wagiran^b ; ^{alina@fbb.utm.my" class="auth_mail" title="E-mail the corresponding author} ; Azman Abd Samad^b ; Zaidah Rahmat^b ; Mohamad Roji Sarmidi^a ; ^c
• 关键词：2 ; 4-D ; 2 ; 4-dichlorophenoxyacetic acid ; NAA ; naphthaleneacetic acid ; Kin ; kinetin ; MS ; Murashige and Skoog ; BAP ; benzylaminopurine ; TDZ ; thidiazuron
• 刊名：Saudi Journal of Biological Sciences
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：23
• 期：1_supp_S
• 页码：S69-S77
• 全文大小：2419 K

文摘

A new and rapid protocol for optimum callus production and complete plant regeneration has been assessed in Malaysian upland rice (Oryza sativa) cv. Panderas. The effect of plant growth regulator (PGR) on the regeneration frequency of Malaysian upland rice (cv. Panderas) was investigated. Mature seeds were used as a starting material for callus induction experiment using various concentrations of 2,4-D and NAA. Optimal callus induction frequency at 90% was obtained on MS media containing 2,4-D (3 mg L⁻¹) and NAA (2 mg L⁻¹) after 6 weeks while no significant difference was seen on tryptophan and glutamine parameters. Embryogenic callus was recorded as compact, globular and light yellowish in color. The embryogenic callus morphology was further confirmed with scanning electron microscopy (SEM) analysis. For regeneration, induced calli were treated with various concentrations of Kin (0.5–1.5 mg L⁻¹), BAP, NAA and 0.5 mg L⁻¹ of TDZ. The result showed that the maximum regeneration frequency (100%) was achieved on MS medium containing BAP (0.5 mg L⁻¹), Kin (1.5 mg L⁻¹), NAA (0.5 mg L⁻¹) and TDZ (0.5 mg L⁻¹) within four weeks. Developed shoots were successfully rooted on half strength MS free hormone medium and later transferred into a pot containing soil for acclimatization. This cutting-edge finding is unique over the other existing publishable data due to the good regeneration response by producing a large number of shoots.