Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during in vitro shoot regeneration of Nicotiana tabacum callus: Implications for bioprocess routes to plant-made biopharmaceuticals

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• 作者：Sherwin S. Barretto^a ; ^{biotech2099@gmail.com} ; Franck Michoux^c ; Klaus Hellgardt^b ; Peter J. Nixon^a
• 关键词：CIM ; callus induction medium ; kDa ; kiloDalton ; MS medium ; Murashige & Skoog medium ; RITA® ; recipient for automated temporary immersion (translated from French) ; SDS-PAGE ; sodium dodecyl sulphate polyacrylamide gel electrophoresis ; TetC ; fragment C of tetanus toxin ; TF ; triphenylformazan ; TIB ; temporary immersion bioreactor ; TSP ; total soluble protein ; TTC ; 2 ; 3 ; 5-triphenyltetrazolium chloride
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：117
• 期：part_PB
• 页码：73-81
• 全文大小：1881 K
• 卷排序：117

文摘

Tissue culture is advantageous for ‘molecular farming’ in transplastomic plants. In vitro shoot regeneration of N. tabacum callus was carried out. Biological responses were correlated to pneumatic hydrodynamic parameters. Reduced growth and mitochondrial activity occurred above a critical shear rate. Transplastomic protein yield declined exponentially with increasing aeration.