Regulation of toll-like receptors 3, 7 and 9 in porcine alveolar macrophages by different genotype 1 strains of porcine reproductive and respiratory syndrome virus

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• 作者：Liudmila Kuzemtseva^a ; ¹ ; Eugenia de la Torre^a ; ¹ ; Gerard Martí ; n^a ; Ferran Soldevila^a ; Tahar Ait-Ali^b ; Enric Mateu^a ; ^c ; Laila Darwich^a ; ^c ; ^{laila.darwich@uab.cat" class="auth_mail}
• 关键词：Genotype 1 PRRSV ; Toll-like receptors ; Porcine alveolar macrophages ; Innate immunity
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：15 April, 2014
• 年：2014
• 卷：158
• 期：3-4
• 页码：189-198
• 全文大小：1390 K

文摘

The toll-like receptors (TLRs) play an important role in the innate host defense against pathogens. Endosomal TLRs, TLR3, TLR7/8, and TLR9 are involved in antiviral responses by promoting the production of antiviral cytokines such as type I interferons. Porcine reproductive and respiratory syndrome (PRRS) is an important disease causing economically high losses to the swine industry worldwide and caused by a single stranded positive sense RNA virus, known as PRRS virus (PRRSV). Studies focused on the interaction between PRRSV and TLRs are scarce. The aim of the present study was to evaluate the expression of TLR3, TLR7 and TLR9 in porcine alveolar macrophages (PAM) infected with different genotype 1 PRRSV strains previously sequenced and characterized by their ability to induce TNF-伪: 3262 (TNF-伪 inducer), 3267 (TNF-伪 not inducer) and an attenuated vaccine strain (strain Deventer, Porcilis^庐PRRS, Merck) that replicates scarcely in PAM. PAM were infected with the different PRRSV strains (at 0.1 multiplicity of infection) for 48 h or mock-stimulated with PAM supernatants. Cells were collected at different time-points (0 h, 6 h, 12 h, 24 h, 36 h, 48 h) to determine the kinetics of viral replication by quantitative RT-PCR (qRT-PCR) and the expression of TLR3, 7 and 9 by qRT-PCR, flow cytometry and indirect immunofluorescence assay. Although infection with PRRSV did not affect significantly relative levels of any TLR mRNA transcript (normalized to 尾-actin expression), this infection resulted in significant differences in the proportion of cells expressing TLR3. Thus, in PAM infected with PRRSV strain 3262 the proportion of TLR3+ cells significantly increased from 24 h compared with the controls; in contrast strain 3267 resulted in a lower proportion of TLR3+ PAM. Interestingly, strain 3262 replicate to lower levels than 3267 at comparable post-inoculation times. For strain DV, the results indicated that this strain did not replicate substantially in PAM and did not stimulated TLR3 expression. These observations suggest that at least TLR3 is regulated differentially by different genotype 1 PRRSV strains and this seems to be related apparently to the replication levels of each strain, as well as, to the TNF-伪 inducing capability. The fact that mRNA transcripts were kept constant also suggests that this regulation occurs at a post-transcriptional level.