- 作者：Moussa Labsi ; Lila Khelifi ; Dalila Mezioug ; Imene Soufli ; Chafia Touil-Boukoffa ; touilboukoffa@yahoo.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：PGE ; Tumor necrosis factor-α ; iNOS ; Nuclear factor-κB
- 刊名：Asian Pacific Journal of Tropical Medicine
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：9
- 期：3
- 页码：211-220
- 全文大小：2249 K
Methods
Swiss mice were inoculated intraperitoneally with viable protoscoleces. Then, PGE was orally administered daily during cystic echinococcosis development. Cyst development and hepatic damage were macroscopically and histologically analyzed. The production of nitric oxide and TNF-α was assessed in plasma and the hepatic expression of iNOS, TNF-α, NF-κB and CD68 was examined. Moreover, protoscoleces were cultured and treated with different concentrations of PGE.
Results
It was observed that in vitro treatment of protoscoleces caused a significant decrease in viability in a PGE-dose-dependent manner. In vivo, after treatment of cystic echinococcosis infected mice with PGE, a significant decrease in nitric oxide levels (P < 0.000 1) and TNF-α levels (P < 0.001) was observed. This decline was strongly related to the inhibition of cyst development (rate of hydatid cyst growth inhibition = 63.08%) and a decrease in CD68 expression in both the pericystic layer of hepatic hydatid cysts and liver tissue (P < 0.000 1). A significant diminution of iNOS, TNF-α and NF-κB expression was also observed in liver tissue of treated mice (P < 0.000 1).
Conclusions
Our results indicate an antihydatic scolicidal effect and immunomodulatory properties of PGE, suggesting its potential therapeutic role against Echinococcus granulosus infection.