Dengue virus infection-enhancing antibody activities against Indonesian strains in inhabitants of central Thailand

详细信息    查看全文

• 作者：Atsushi Yamanaka^a ; ^b ; ^{knmya@biken.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Duangjai Oddgun^a ; ^b ; Nantarat Chantawat^c ; ^d ; Tamaki Okabayashi^c ; ^d ; Pongrama Ramasoota^e ; ^{pongrama.ram@mahidol.ac.th" class="auth_mail" title="E-mail the corresponding author} ; Siti Churrotin^f ; Tomohiro Kotaki^f ; ^g ; Masanori Kameoka^g ; Soegeng Soegijanto^f ; Eiji Konishi^a ; ^b
• 关键词：Dengue ; Antibody-dependent enhancement ; Viral antigens ; Recombinant proteins ; Indonesia ; Thailand
• 刊名：Microbes and Infection
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：18
• 期：4
• 页码：277-284
• 全文大小：515 K

文摘

Dengue virus (DENV) infection-enhancing antibodies are a hypothetic factor to increase the dengue disease severity. In this study, we investigated the enhancing antibodies against Indonesian strains of DENV-1–4 in 50 healthy inhabitants of central Thailand (Bangkok and Uthai Thani). Indonesia and Thailand have seen the highest dengue incidence in Southeast Asia. The infection history of each subject was estimated by comparing his/her neutralizing antibody titers against prototype DENV-1–4 strains. To resolve the difficulty in obtaining foreign live viruses for use as assay antigens, we used a recombinant system to prepare single-round infectious dengue viral particles based on viral sequence information. Irrespective of the previously infecting serotype(s), most serum samples showed significantly higher enhancement titers against Indonesian DENV-2 strains than against Thai DENV-2 strains, whereas the opposite effect was observed for the DENV-3 strains. Equivalent enhancing activities were observed against both DENV-1 and DENV-4. These results suggest that the genotype has an impact on enhancing antibody activities against DENV-2 and DENV-3, because the predominant circulating genotypes of each serotype differ between Indonesia and Thailand.