Enhanced efficacy and anti-biofilm activity of novel nanoemulsions against skin burn wound multi-drug resistant MRSA infections

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• 作者：Zhen Song ; Hongwu Sun ; PhD ; ^{sunhongwu2001@163.com" class="auth_mail" title="E-mail the corresponding author} ; Yun Yang ; BA ; Haiming Jing ; MD ; Liuyang Yang ; BA ; Yanan Tong ; BA ; Chao Wei ; BA ; Zelin Wang ; Quanming Zou ; PhD ; Hao Zeng ; PhD ; ^{zeng1109@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Nanoemulsion ; Chlorhexidine acetate ; Methicillin-resistant Staphylococcus aureus ; Anti-biofilm activity ; Burn wound skin infection mouse model
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：12
• 期：6
• 页码：1543-1555
• 全文大小：4403 K

文摘

Multi-drug resistant MRSA (methicillin-resistant Staphylococcus aureus) is a global problem for human health, especially skin burn wound patients. Therefore, we estimated the antibacterial and anti-biofilm activity of a chlorhexidine acetate nanoemulsion (CNE) by previously ourselves designed against skin burn wound MRSA infections. Compared with its water solution (CHX), CNE showed a better and faster action against MRSA both in vitro and in vivo. Importantly, CNE was more effective at inhibiting biofilm formation and clearing the biofilm. We also found that the cell walls and membranes of MRSA were severely disrupted after treatment with CNE. Moreover, the relative electrical conductivity and the leakage of alkaline phosphates, K⁺, Mg^2 +, DNA and protein obviously increased because the cell wall and membrane were damaged. These data show that novel CNE is a promising potential antimicrobial candidate, especially for skin burn wound MRSA infections.