Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A
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- 作者：Zhenhai Chena ; 1 ; Fangfeng Yuana ; b ; 1 ; Yanhua Lia ; 1 ; Pengcheng Shanga ; Robin Schroederc ; Kelly Lechtenbergc ; Jamie Henningsona ; b ; Benjamin Hausea ; b ; Jianfa Baia ; b ; Raymond R.R. Rowlanda ; Alfonso Clavijoa ; b ; Ying Fanga ; b ; yfang@vet.k-state.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Senecavirus A ; Picornavirus infection ; Infectious clone ; Reverse genetics ; EGFP reporter virus ; Swine ; Vesicular lesion ; Vesicular disease ; SVA pathogenesis
- 刊名：Virology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：497
- 期：Complete
- 页码：111-124
- 全文大小：3330 K
文摘
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An infectious clone, pKS15-01-Clone, of newly emerging strain of SVA was constructed.
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Cloned virus replicated efficiently in vitro and in vivo.
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Cloned virus induced similar immunological response as that of parental virus.
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EGFP-tagged SVA showed impaired replication ability and attenuated pathogenic properties.
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Lesions on pig snout and coronary bands produced by parental virus, but not by cloned or EGFP-tagged virus.