Protective and immunogenic effects of Escherichia coli-expressed infectious pancreatic necrosis virus (IPNV) VP2-VP3 fusion protein in rainbow trout
详细信息 查看全文
- 作者：Maryam Dadara ; dadar.m77@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Hamid Rajabi Memarib ; Vikram N. Vakhariac ; Rahim Peyghana ; MasodReza Seifi Abad Shapourid ; Takavar Mohammadiana ; Reza Hasanzadehe ; Mohades Ghasemif
- 关键词：Infectious pancreatic necrosis virus (IPNV) ; VP2-VP3 fusion protein ; E. coli expression ; Recombinant vaccine ; Rainbow trout
- 刊名：Fish & Shellfish Immunology
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：47
- 期：1
- 页码：390-396
- 全文大小：840 K
文摘
- •
IPNV VP2-VP3 fusion gene was cloned into a pET-26b expression vector and recombinant protein was produced in E. coli host.
- •
E. coli-expressed IPNV VP2-VP3 fusion protein (vaccine) was intraperitoneally administered to rainbow trout.
- •
The recombinant IPNV vaccine induced strong immune responses and protected vaccinated fish against IPNV challenge.
- •
The relative percent survival rate (RPS) of survivors was 83% for the vaccinated group.
- •
E. coli-expressed IPNV vaccine significantly reduced virus load in tissues of the vaccinated fish.