A hemagglutinin-esterase-expressing salmonid alphavirus replicon protects Atlantic salmon (Salmo salar) against infectious salmon anemia (ISA)

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• 作者：Astrid Wolf^a ; ^{astrid.wolf@nvh.no} ; Kjartan Hodneland^b ; ^{kjartan.hodneland@merck.com} ; Petter Frost^b ; ^{petter.frost@merck.com} ; Stine Braaen^a ; ^{stine.braaen@nvh.no} ; Espen Rimstad^a ; ^{espen.rimstad@nvh.no}
• 关键词：Salmonid alphavirus-based replicon vaccine ; Immunization against ISA ; Infectious salmon anemia virus ; Alphavirus replicon vector ; Reverse genetic system salmonid alphavirus
• 刊名：Vaccine
• 出版年：2013
• 出版时间：11 January, 2013
• 年：2013
• 卷：31
• 期：4
• 页码：661-669
• 全文大小：797 K

文摘

A replicon expression system based on the salmonid alphavirus (SAV) that encodes the infectious salmon anemia virus (ISAV) hemagglutinin-esterase (HE) was constructed and found to be an efficacious vaccine against infectious salmon anemia (ISA). Following a single intramuscular immunization, Atlantic salmon (Salmo salar) were effectively protected against subsequent ISAV challenge. Additional replicons coding for the ISAV fusion glycoprotein (F) or the ISAV matrix protein (M) were created and tested in combination with the replicon that encodes the HE. The ISAV HE was confirmed as a potent antigen, but neither the F nor the M proteins were found to be essential for immunization-induced protection. Innate immune response induced at the site of vaccination illustrated the immunogenicity of the SAV-based replicon and its ability to activate antiviral responses in Atlantic salmon. The successful testing of the SAV-based replicon as a vaccine model against ISA showed that the replicon approach may represent a novel immunization technology for the aquaculture industry. It offers potential benefits in terms of safety, efficacy, flexibility, and vaccine production complexity.