Mushroom lectin enhanced immunogenicity of HBV DNA vaccine in C57BL/6 and HBsAg-transgenic mice

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• 作者：Wenjuan Gao^a ; Yuhan Sun^a ; Shiwen Chen^a ; Jingyao Zhang^a ; Jingjing Kang^b ; Yongqiang Wang^c ; Hexiang Wang^a ; Guoliang Xia^a ; Qinghong Liu^a ; ^{qhliu@cau.edu.cn} ; Youmin Kang^a ; ^{kym@cau.edu.cn} ; ^{kymin1012@163.com}
• 关键词：HBV ; hepatitis B virus ; POL ; lectin purified from pleurotus ostreatus ; HBVsAg-Tg ; HBV surface antigen transgenic ; DTH ; delayed-type hypersensitivity ; HBVsAb ; HBV surface protein antibody ; PZQ ; praziquantel ; rHBVsAg ; recombinant HBV surface antigen ; MTT ; 3
• 刊名：Vaccine
• 出版年：2013
• 出版时间：26 April, 2013
• 年：2013
• 卷：31
• 期：18
• 页码：2273-2280
• 全文大小：1626 K

文摘

DNA vaccination is a promising strategy for activating immune responses against hepatitis B virus (HBV) infection. However, the accumulated data have shown that DNA vaccination alone generates weak immune responses. To enhance the immunogenicity of HBV DNA vaccine, lectin purified from pleurotus ostreatus (POL) was used as adjuvant of HBV DNA vaccine for C57BL/6 and HBV surface antigen transgenic (HBVsAg-Tg) mice. Our data demonstrate that low dose of POL (1 ¦Ìg/mouse) in conjunction with HBV DNA vaccine stimulated stronger HBV-specific delayed-type hypersensitivity (DTH) responses and higher HBV-specific IgG level than that in high dose of POL groups (5 ¦Ìg/mouse and 10 ¦Ìg/mouse). POL activated strong Th2 and Tc1 cell responses in immunized C57BL/6 and HBVsAg-Tg mice. POL as adjuvant of HBV DNA vaccine effectively enhanced HBV surface protein antibody (HBVsAb) and decreased HBVsAg level for HBV Tg mice treatment. Furthermore, POL infiltrated more lymphocytes excluding Th1, Th2 and Tc1 cell subtypes to liver of HBVsAg-Tg mice. Together, these results suggest that POL as adjuvant enhanced immunogenicity of HBV DNA vaccination and effectively stimulated immune reaponse for HBsAg-Tg mice treatment. Our findings implicate the potential of mushroom lectin as adjuvant of HBV DNA vaccine.