Effectiveness of hydrogen peroxide and electron-beam irradiation treatment for removal and inactivation of viruses in equine-derived xenografts
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- 作者：Riccardo Cusinatoa ; Monia Pacentia ; monia.pacenti@sanita.padova.it" class="auth_mail" title="E-mail the corresponding author ; Thomas Martelloa ; thomas.martello@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Paolo Fattorib ; p.fattori@bioteck.biz" class="auth_mail" title="E-mail the corresponding author ; Marco Morronib ; m.morroni@bioteck.com" class="auth_mail" title="E-mail the corresponding author ; Giorgio Palù ; a ; giorgio.palu@unipd.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cox-B1 ; Coxsackievirus B1 ; CPE ; Cytopathic Effect ; DMEM ; Dulbecco&rsquo ; s Modified Eagle Medium ; dsDNA ; double-stranded DNA ; EQA ; External Quality Assessment ; FBS ; Fetal Bovine Serum ; FDA ; Food and Drugs Administration ; H1N1 ; influenza A virus subtype H1N1 ; HSV-1 ; Herpes simplex virus type 1 ; MDCK ; Madin-Darby Canine Kidney ; PBS ; Phosphate Buffered Saline ; qPCR ; quantitative Real-Time PCR ; RT-qPCR ; Reverse-Transcriptase qPCR ; SAL ; Sterility Assurance Level ; ssRNA ; single-stranded RNA ; TCID50 ; Ti
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：232
- 期：Complete
- 页码：39-46
- 全文大小：883 K
文摘
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Almost no viral inactivation studies have been performed on equine-derived grafts.
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Hydrogen peroxide and e-beam radiation are used in the manufacture of xenografts.
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Combined hydrogen peroxide and e-beam irradiation showed synergistic antiviral effects.
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There was >4 log viral titre reduction in all cases.