Integrated microfluidic device using a single universal aptamer to detect multiple types of influenza viruses
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- 作者：Chih-Hung Wanga ; Chih-Peng Changb ; Gwo-Bin Leea ; c ; d ; gwobin@pme.nthu.edu.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ca++ ; Calcium ion ; ddH2O ; Double-distilled water ; FAM ; Carboxyfluorescein ; HA ; Hemagglutination ; InfA ; Influenza A virus ; InfB ; Influenza B virus ; K+ ; Potassium ion ; LOD ; Limit of detection ; Mg++ ; Magnesium ion ; Na+ ; Sodium ion ; NC ; Negative control ; PBS ; Phosphate-buffered saline ; PDMS ; Polydimethylsiloxane ; PMT ; Photo-multiplier tube ; RNA ; Ribonucleic acid ; RT-PCR ; Reverse transcriptase polymerase chain reaction ; SELEX ; Systematic evolution of ligands and exponential enrichment
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：247-254
- 全文大小：1598 K
文摘
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A new integrated microfluidic system was developed to detect multiple types of the influenza virus.
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The change in conformation of a single universal influenza aptamer exposed to different ion concentrations could detect three different influenza viruses.
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Fast diagnosis of influenza A H1N1, H3N2 and influenza B could be automated on an integrated microfluidic system within 30 min by using a single universal aptamer exposed to different ion concentrations.