Dosage comparison of Congo Basin and West African strains of monkeypox virus using a prairie dog animal model of systemic orthopoxvirus disease

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• 作者：Christina L. Hutson ; Darin S. Carroll ; Joshua Self ; Sonja Weiss ; Christine M. Hughes ; Zachary Braden ; Victoria A. Olson ; Scott K. Smith ; Kevin L. Karem ; Russell L. Regnery ; Inger K. Damon
• 关键词：Monkeypox ; Orthopox ; Pathogenesis
• 刊名：Virology
• 出版年：2010
• 出版时间：20 June 2010
• 年：2010
• 卷：402
• 期：1
• 页码：72-82
• 全文大小：1486 K

文摘

The prairie dog is valuable for the study of monkeypox virus (MPXV) virulence and closely resembles human systemic orthopoxvirus disease. Herein, we utilize a variable dose intranasal challenge with approximately 10³, 10⁴, 10⁵, and 10⁶ PFU for each clade to further characterize virulence differences between the two MPXV clades. A trend of increased morbidity and mortality as well as greater viral shedding was observed with increasing viral challenge dose. Additionally, there appeared to be a delay in onset of disease for animals challenged with lower dosages of virus. Mathematical calculations were used to determine LD₅₀ values and based on these calculations, Congo Basin MPXV had approximately a hundred times lower LD₅₀ value than the West African clade (5.9 × 10³ and 1.29 × 10⁵ respectively); reinforcing previous findings that Congo Basin MPXV is more virulent.