Genotype-dependent sex differentiation of dopaminergic neurons in primary cultures of embryonic mouse brain

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• 作者：Sibug ; Rosana ; Kü ; ppers ; Eva ; Beyer ; Cordian ; Maxson ; Stephen C. ; Pilgrim ; Christof ; Reisert ; Ingrid
• 关键词：Inter-strain variability ; Sex difference ; Culture ; Genotype ; Sex steroid ; Dopamine uptake ; HPLC ; Tyrosine hydroxylase immunocytochemistry
• 刊名：Developmental Brain Research
• 出版年：1996
• 出版时间：May 31, 1996
• 年：1996
• 卷：93
• 期：1-2
• 页码：136-142
• 全文大小：560 K

文摘

In order to investigate genetic factors that interfere with hormone-mediated sex differentiation of dopaminergic neurons, we raised sex-specific primary cultures from embryonic day 13 diencephalon (D) or mesencephalon (M) of three different strains of mice, NMRI, CBA/J, and BALBc/J. Part of the cultures were maintained for 6 or 13 days in vitro (DIV) in medium containing 17β-estradiol or testosterone. The cultures were analyzed for sex differences in numbers of tyrosine hydroxylase-immunoreactive neurons, endogenous dopamine (DA) levels, and specific uptake of [³H]DA. Previous results obtained with cultures of embryonic Sprague-Dawley rats had shown that these parameters develop sex-specific characteristics in the absence of sex differences in hormone environment. Similar steroid-independent sex differences as they occur in the rat were found in M cultures of NMRI but not in CBA and BALBc mice. Long-term sex steroid treatment did not affect any of the abvve parameters in any strain. It is concluded that cell-autonomous realization of the genetic sex of dopaminergic neurons depends on the genetic background.