Ontogenetic expression and splicing of estrogen receptor-α and β mRNA in the rat midbrain

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• 作者：Raab ; Heike ; Karolczak ; Magdalena ; Reisert ; Ingrid ; Beyer ; Cordian
• 关键词：Midbrain ; Development ; Estrogen receptor-α ; /β ; Reverse transcription-polymerase chain reaction ; Splicing ; Dopamine
• 刊名：Neuroscience Letters
• 出版年：1999
• 出版时间：November 5, 1999
• 年：1999
• 卷：275
• 期：1
• 页码：21-24
• 全文大小：94.8 K

文摘

Several studies have shown that estrogen is important for the differentiation of midbrain dopaminergic neurons. This is supported by the previous demonstration of estrogen synthesis in the perinatal ventral midbrain. The present study attempts to characterize the expression pattern of nuclear estrogen receptors (ER-α/β) mRNAs in the ventral rat midbrain during development. By applying primers specific for the hormone-binding domain, ER-α mRNA was detected from embryonic day (E) 14 until postnatal day (P) 20, whereas considerable levels of ER-β mRNA were found from P3 to P20. In contrast, primers spanning the DNA-binding domain demonstrated the presence of transcripts for ER-α as well as ER-β after birth. These findings indicate that both ERs are expressed in the developing midbrain. The presence of ER-α transcripts devoid of the DNA-binding region is discussed in the context of ‘non-genomic’ estrogen signaling possibly by membrane receptors.