Quinazolinone derivatives: Synthesis and comparison of inhibitory mechanisms on α-glucosidase

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• 作者：Mankun Wei^a ; Wei-Ming Chai^a ; ^b ; ^{chaiweiming@jxnu.edu.cn} ; Rui Wang^a ; Qin Yang^a ; ^b ; Zhihong Deng^b ; Yiyuan Peng^a ; ^b ; ^{yypeng@jxnu.edu.cn}
• 关键词：Quinazolinone ; α-Glucosidase ; Fluorescence ; Docking
• 刊名：Bioorganic & Medicinal Chemistry
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：25
• 期：4
• 页码：1303-1308
• 全文大小：2307 K
• 卷排序：25

文摘

In this study, eight quinazolinone derivatives were designed and synthesized. Their inhibitory activities on α-glucosidase were assessed in vitro. Two compounds: 2-(4-chlorophenyl)-quinazolin-4(3H)-one (CQ) and 2-(4-bromophenyl)-quinazolin-4(3H)-one (BQ) were found to be potent inhibitors of α-glucosidase with IC50 values of 12.5 ± 0.1 μM and 15.6 ± 0.2 μM, respectively. Spectroscopy methods were performed to analyze the inhibitory mechanisms of both compounds on α-glucosidase. The results revealed that they reversibly inhibited α-glucosidase in a non-competitive manner. CQ and BQ could statically quench the fluorescence spectra by formation of an inhibitor-α-glucosidase complex. The interaction between CQ and α-glucosidase depended on hydrogen bonds, electrostatic and hydrophobic force, while the driving force of the binding between BQ and the enzyme was hydrophobic. The docking results showed that BQ was less active than CQ against α-glucosidase because of its weaker interaction with the enzyme. In brief, the quinazolinone derivatives identified in this work were potentially promising candidates for developing as novel anti-diabetic agents.