Dual signal amplification of surface plasmon resonance imaging for sensitive immunoassay of tumor marker

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• 作者：Weihua Hu^a ; ^b ; ^c ; ^{whhu@swu.edu.cn" class="auth_mail} ; Hongming Chen^a ; ^b ; Zhuanzhuan Shi^a ; ^b ; Ling Yu^a ; ^b
• 关键词：Surface plasmon resonance imaging (SPRi) ; Gold nanoparticle ; Atom transfer radical polymerization ; Signal amplification ; Tumor marker
• 刊名：Analytical Biochemistry
• 出版年：15 May, 2014
• 年：2014
• 卷：453
• 期：Complete
• 页码：16-21
• 全文大小：1388 K

文摘

Surface plasmon resonance imaging (SPRi) is an intriguing technique for immunoassay with the inherent advantages of being high throughput, real time, and label free, but its sensitivity needs essential improvement for practical applications. Here, we report a dual signal amplification strategy using functional gold nanoparticles (AuNPs) followed by on-chip atom transfer radical polymerization (ATRP) for sensitive SPRi immunoassay of tumor biomarker in human serum. The AuNPs are grafted with an initiator of ATRP as well as a recognition antibody, where the antibody directs the specific binding of functional AuNPs onto the SPRi sensing surface to form immunocomplexes for first signal amplification and the initiator allows for on-chip ATRP of 2-hydroxyethyl methacrylate (HEMA) from the AuNPs to further enhance the SPRi signal. High sensitivity and broad dynamic range are achieved with this dual signal amplification strategy for detection of a model tumor marker, 伪-fetoprotein (AFP), in 10% human serum.