Transamniotic stem cell therapy (TRASCET) in a leporine model of gastroschisis
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- 作者：Christina Feng ; Christopher D. Graham ; Hester F. Shieh ; Joseph A. Brazzo III ; John Patrick Connors ; Lucas Rohrer ; Alexander Papadakis ; David Zurakowski ; Dario O. Fauza ; dario.fauza@childrens.harvard.edu
- 关键词：Amniotic mesenchymal stem cells ; Transamniotic stem cell therapy ; Gastroschisis ; Fetal stem cells
- 刊名：Journal of Pediatric Surgery
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：52
- 期：1
- 页码：30-34
- 全文大小：700 K
- 卷排序：52
文摘
Transamniotic stem cell therapy (TRASCET) with amniotic fluid mesenchymal stem cells (afMSCs) has been shown to mitigate bowel damage in a rodent model of gastroschisis. As a prerequisite to clinical translation, we sought to study TRASCET in a larger animal model.MethodsNew Zealand rabbit fetuses (n = 64) with surgically created gastroschisis were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intraamniotic injections of either saline or a suspension of afMSCs. Nonmanipulated fetuses served as controls. Histomorphologic measurements of intestinal damage, along with biochemical profiling of inflammation markers, were performed at term. Statistical comparisons were by Fisher's exact test, ANOVA and the Wald test (P < 0.05).ResultsOverall survival was 62.5%. Segmental and total intestinal wall thicknesses were significantly decreased in the afMSC group compared with the untreated and saline groups (all P < 0.001), with no significant differences between untreated and saline groups (P = 0.24 to 1.00, depending on layer). Muscularis and serosal layers were significantly thicker in the afMSC group than in normal controls (P = 0.045 and P < 0.001, respectively).ConclusionsConcentrated intraamniotic injection of afMSC lessens, yet does not prevent, intestinal damage in a leporine model of gastroschisis. TRASCET may become a valuable strategy in the management of gastroschisis.Level of evidenceN/A - animal/experimental studies.