Inocula selection in microbial fuel cells based on anodic biofilm abundance of Geobacter sulfurreducens

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• 作者：Guotao Sun¹ ; Diogo de Sacadura Rodrigues¹ ; Anders Thygesen¹ ; ^{athy@kt.dtu.dk" class="auth_mail" title="E-mail the corresponding author} ; Geoffrey Daniel² ; Dinesh Fernando² ; Anne S. Meyer¹
• 关键词：Lake sediment ; Coulombic efficiency ; Denaturing gradient gel electrophoresis ; Geobacter sulfurreducens ; Anode polarisation resistance
• 刊名：Chinese Journal of Chemical Engineering
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：24
• 期：3
• 页码：379-387
• 全文大小：1592 K

文摘

Microbial fuel cells (MFCs) rely on microbial conversion of organic substrates to electricity. The optimal performance depends on the establishment of a microbial community rich in electrogenic bacteria. Usually this microbial community is established from inoculation of the MFC anode chamber with naturally occurring mixed inocula. In this study, the electrochemical performance of MFCs and microbial community evolution were evaluated for three inocula including domestic wastewater (DW), lake sediment (LS) and biogas sludge (BS) with varying substrate loading (L_sub) and external resistance (R_ext) on the MFC. The electrogenic bacterium Geobacter sulfurreducens was identified in all inocula and its abundance during MFC operation was positively linked to the MFC performance. The LS inoculated MFCs showed highest abundance (18% ± 1%) of G. sulfurreducens, maximum current density [I_max = (690 ± 30) mA·m^− 2] and coulombic efficiency (CE = 29% ± 1%) with acetate as the substrate. I_max and CE increased to (1780 ± 30) mA·m^− 2 and 58% ± 1%, respectively, after decreasing the R_ext from 1000 Ω to 200 Ω, which also correlated to a higher abundance of G. sulfurreducens (21% ± 0.7%) on the MFC anodic biofilm. The data obtained contribute to understanding the microbial community response to L_sub and R_ext for optimizing electricity generation in MFCs.