Characterization and pathogenicity of acute hepatopancreatic necrosis disease natural mutants, pirAB_vp (‐) V. parahaemolyticus, and pirAB_vp (+) V. campbellii strains

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• 作者：J.E. Han^a ; ^{hanje1223@gmail.com} ; K.F.J. Tang^a ; L.F. Aranguren^a ; P. Piamsomboon^b
• 关键词：Composite transposon ; Early mortality syndrome ; Gene deletion ; Horizontal gene transfer ; Insertion sequence (IS) ; Tn6264
• 刊名：Aquaculture
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：470
• 期：Complete
• 页码：84-90
• 全文大小：1242 K
• 卷排序：470

文摘

A composite transposon, named Tn6264, consists of pirABvp genes and the flanking insertion sequence (ISVal1), is associated with AHPND of shrimp. Two types of pirABvp deletion mutants of V. parahaemolyticus were found in the AHPND-affected farms. The type I mutants, named pirABvp(-), have deletions (4.4-kb or 6.0-kb) of entire pirABvp genes and the downstream ISVal1. The type II mutants, named pirAvp(-), have smaller deletions (1.5-kb or 1.6-kb) of pirAvp and including a partial pirBvp. V. campbellii strains carrying pirABvp genes were found, through laboratory bioassays and histologicial evaluation, to cause AHPND.