文摘
The N2A-IS region contains 15% α-helix. 2,2,2-Trifluoroethanol induces additional helical character in N2A-IS. The 13.9 kDa N2A-IS migrates as a 45 kDa protein on size exclusion chromatography. N2A-IS does not exhibit cooperative unfolding or folding. N2A-IS has a mean end-to-end distance of 5.2 nm as measured by FRET.