We have developed a protocol that consistently produces de novo chronic graft-versus-host disease in the clinically relevant hematopoietic cell transplantation canine model We have shown that the esophagus is targeted in the canine chronic graft-versus-host disease in a manner similar to that of the human We have shown that the outbred canine model possesses a broad spectrum of manifestations of chronic graft-versus-host disease that are typically seen clinically Initial mechanistic studies revealed that levels of select chemokines and cytokines are similarly affected in the canine as in the clinical setting