- 作者：Xiao-jin ZHANG ; Sui-qi GUI ; Lin CAO ; Zu-yue SUN
- 关键词：trophoblast cells ; insuline-like growth factor-I ; progesterone ; low density lipoprotein receptor (LDLR)
- 刊名：Journal of Reproduction and Contraception
- 出版年：2007
- 出版时间：December 2007
- 年：2007
- 卷：18
- 期：4
- 页码：237-246
- 全文大小：264 K
Objective
To investigate the effect of insuline-like growth factor-I (IGF-I) on progesterone genesis and regulation.Methods
Cytotrophoblast cells were collected by trypsin-collagenase digestion and percoll gradient centrifugation for primary culture. After stimulated with different concentrations(100 μg/ml, 10 μg/ml, 1 μg/ml, 0.1 μg/ml) of IGF-I at the same time and with different duration(12 h, 24 h, 48 h, 72 h) of IGF-I with the same concentration, progesterone levels in the media were measured by radioimmunoassay. Simultaneously, semiquantitative reverse transcriptase polymerase chain reaction(RT-PCR) was applied to determine the expression of low density lipoprotein receptor (LDLR) mRNA.
Results
Progesterone levels correlated positively with IGF-I along with the IGF-I concentration increasing, progesterone level began to increase at 12 h, and reached the climax at 48 h when cultured with 100 µg/L IGF-I. The expression of LDLR mRNA was detectable in every group and accordant with variation of progesterone level.
Conclusion
Progesterone secretion has time- and dose-dependent effect on IGF-I, and IGF-I can up-regulate the expression of LDLR mRNA. IGF-I may play an important role in promoting secretion of progesterone in trophoblast cells.