Escherichia coli and Pseudomonas aeruginosa eradication by nano-penicillin G

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• 作者：Margarida M. Fernandes ; PhD^a ; Kristina Ivanova ; MSc^a ; Antonio Francesko ; PhD^a ; Diana Rivera ; MSc^a ; Juan Torrent-Burgué ; s ; PhD^a ; Aharon Gedanken ; PhD^b ; Ernest Mendonza ; PhD^c ; Tzanko Tzanov ; PhD^a ; ^{tzanko.tzanov@upc.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Penicillin G ; Sonochemistry ; Nano/Microspheres ; Nano-bio interactions ; Antibacterial ; Antibiofilm
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：12
• 期：7
• 页码：2061-2069
• 全文大小：1155 K

文摘

The transformation of penicillin G into nano/micro-sized spheres (nanopenicillin) using sonochemical technology was explored as a novel tool for the eradication of Gram-negative bacteria and their biofilms. Known by its effectiveness only against Gram-positive microorganisms, the penicillin G spherization boosted the inhibition of the Gram-negative Pseudomonas aeruginosa 10-fold (from 0.3 to 3.0 log-reduction) and additionally induced 1.2 log-reduction of Escherichia coli growth. The efficient penetration of the spheres within a Langmuir monolayer sustained the theory that nanopenicillin is able to cross the membrane and reach the periplasmic space in Gram-negative bacteria where they inhibit the β-lactam targets: the transferases that build the bacteria cell wall. Moreover, it considerably suppressed the growth of both bacterial biofilms on a medically relevant polystyrene surface, leaving majority of the adhered cells dead compared to the treatment with the non-processed penicillin G. Importantly, nanopenicillin was found innocuous towards human fibroblasts at the antibacterial-effective concentrations.