Single-molecule sorting of DNA helicases
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- 作者：Fletcher E. Bain1 ; Colin G. Wu1 ; Maria Spies ; maria-spies@uiowa.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ATP ; adenosine triphosphate ; BAP ; biotin acceptor peptide ; bio ; biotinylated ; BLM ; Bloom&rsquo ; s syndrome DNA helicase ; BRC1 ; Breast cancer type 1 protein ; BRCT ; BRCA1 C-terminus ; DMEM ; Dulbecco&rsquo ; s modified eagle medium ; DNA ; deoxyribonucleic acid ; ds ; double-stranded ; DPBS ; Dubelcco&rsquo ; s phosphate buffered saline ; DPSS ; Diode pumped solid state ; DTT ; dithiothreitol ; EDTA ; ethylenediaminetetraacetic acid ; EMCCD ; electon multiplying charge coupled device ; FANCJ ; Fanconi anemia complementa
- 刊名：Methods
- 出版年：2016
- 出版时间：1 October 2016
- 年：2016
- 卷：108
- 期：Complete
- 页码：14-23
- 全文大小：2188 K
文摘
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Posttranslational modifications alter activities and interactions of DNA helicases.
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Helicases with native modifications are produced and biotinylated in human cells.
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Single-molecule analyses is performed on surface-tethered helicases.
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Single-molecule sorting separates activities of modified and unmodified helicases.
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Relative abundance of the modification is quantified.