Real-time label-free detection and kinetic analysis of Etanercept—Protein A interactions using quartz crystal microbalance

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• 作者：Evan A. Dubiel^a ; ^b ; Bruno Martin^a ; Sylvain Vigier^a ; ^b ; Patrick Vermette^a ; ^b ; ^{Patrick.Vermette@USherbrooke.ca}
• 关键词：Quartz crystal microbalance (QCM) ; Ligand-receptor interactions ; Biologics ; Interaction kinetics ; Biosensors ; Biopharmaceuticals
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：149
• 期：Complete
• 页码：312-321
• 全文大小：2134 K
• 卷排序：149

文摘

Quartz crystal microbalance, a real-time label-free analysis platform for the biopharma. Enbrel-Protein A interaction kinetics is modeled by the Langmuir binding model. Association/dissociation rate constants decrease as Enbrel concentration increases. The decrease of rate constants is a consequence of mass transport limitations. QCM dissipation measurements provide insight on mass transport limitations.