A site-directed mutagenesis study of the MdmX RING domain
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- 作者：Olga Egorova ; Yi Sheng ; yisheng@yorku.ca" class="auth_mail
- 关键词：Mdm ; Murine Double Minute ; RING ; Really Interesting New Gene
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：16 May 2014
- 年：2014
- 卷：447
- 期：4
- 页码：696-701
- 全文大小：1896 K
文摘
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This study is a comparative structural analysis of the Mdm2 and MdmX RING domains.
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The Mdm2 RING domain residues were introduced into the MdmX RING domain.
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Two mutations N448C and K478R turned MdmX RING into an active E3 ligase.
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Mdm2 residues C449 and R479 are critical for Mdm2 mediated ubiquitination.