Enhancement of n-decane hydroxylation activity of Monilliera sp. NAP 00702 in a liquid-liquid interface bioreactor by mixing of anion-exchange resin microparticles

详细信息    查看全文

• 作者：Shinobu Oda^a ; ^{odas@neptune.kanazawa-it.ac.jp} ; Naoki Sakamoto^a ; Hideo Horibe^b ; Akihiko Kono^b ; Shinichi Ohashi^a
• 关键词：PAN ; polyacrylonitrile ; MS ; ballooned microsphere ; L&ndash ; L IBR ; liquid&ndash ; liquid interface bioreactor ; SmC ; submerged cultivation ; SSC ; solid-state cultivation ; LSI ; liquid-surface immobilization ; Ext-LSI ; extractive liquid-surface immobilization ; IE
• 刊名：Process Biochemistry
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：47
• 期：12
• 页码：2494-2499
• 全文大小：550 K

文摘

Fungal cells immobilized with ballooned polyacrylonitrile (PAN) microsphere (MS) on an interface between a liquid medium and a hydrophobic organic solvent efficiently catalyze various microbial transformations such as hydrolysis, reduction, hydroxylation, and epoxidation of lipophilic substrates dissolved in the organic phase (liquid-liquid interface bioreactor; L-L IBR). In this study, the electric charge of MS layer in the L-L IBR was modified by mixing 4 kinds of anion-exchange, 2 kinds of cation-exchange or a chelating resin microparticles, and applied to regio- and stereoselective subterminal hydroxylation of n-decane to (?)-4-decanol by Monilliera sp. NAP 00702. In general, the addition of cation-exchange and chelating resin microparticles into a PAN-MS layer led to the strong inhibition of fungal growth and hydroxylation activity. On the other hand, the addition of anion-exchange resin microparticles having moderate total capacity (¡Ü1.00 meq/g) significantly increased hyphal growth and hydroxylation activity. Especially, the addition of IRA910CT microparticles (total capacity ¡Ý 1.00 meq/g) into a PAN-MS layer led to 1.6-fold increase of 4-decanol production. Thus, it was concluded that the moderate addition of anion-exchange resin microparticles into an MS layer in the L-L IBR system was effective to enhancement of fungal growth and hydroxylation activity.