Refractive index matching to develop transparent polyaphrons: Characterization of immobilized proteins
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- 作者：Keeran Ward keeran.ward10@ic.ac.uk" class="auth_mail" title="E-mail the corresponding author ; David C. Stuckey ; d.stuckey@ic.ac.uk" class="auth_mail" title="E-mail the corresponding author ; d.stuckey@imperial.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Protein ; Conformation ; Colloidal liquid aphrons (CLAs) ; Refractive index matching ; Adsorption ; Immobilization
- 刊名：Colloids and Surfaces B: Biointerfaces
- 出版年：2016
- 出版时间：1 June 2016
- 年：2016
- 卷：142
- 期：Complete
- 页码：159-164
- 全文大小：1087 K
文摘
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We evaluate the adsorbed structure of four proteins at the oil/water interface.
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Refractive index matching shows proteins are unchanged upon adsorption to the CLA.
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Hydrophobic interactions increase adsorption rate due to the presence of a solvent core.
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Non-ionic surfactants show no interactions with proteins.
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Structural preservation due to bound water within the CLA structure.