Lipid-based transfection reagents can interfere with cholesterol biosynthesis

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• 作者：Mauro Danielli ^{danielli@ifise-conicet.gov.ar" class="auth_mail" title="E-mail the corresponding author} ; Raú ; l A. Marinelli ; ^{marinelli@ifise-conicet.gov.ar" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Transfection reagents ; Cholesterol ; Huh-7 cells
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：15 February 2016
• 年：2016
• 卷：495
• 期：Complete
• 页码：1-2
• 全文大小：306 K

文摘

Lipid-based transfection reagents are widely used for delivery of small interfering RNA into cells. We examined whether the commonly used commercial transfection reagents DharmaFECT-4 and Lipofectamine 2000 can interfere with lipid metabolism by studying cholesterogenesis. Cholesterol de novo synthesis from [¹⁴C]acetate was assessed in human hepatocyte-derived Huh-7 cells. The results revealed that DharmaFECT, but not Lipofectamine, markedly inhibited cholesterol biosynthesis by approximately 70%. Cell viability was not significantly altered. These findings suggest that caution is required in the choice of certain lipid-based transfection reagents for gene silencing experiments, particularly when assessing cholesterol metabolism.