Interferon-¦Â induces a long-lasting antiviral state in human respiratory epithelial cells

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• 作者：Giel R. Gaajetaan^a ; ^c ; Tanja H. Geelen^a ; ^c ; Juanita H. Vernooy^b ; ^c ; Mieke A. Dentener^b ; ^c ; Niki L. Reynaert^b ; ^c ; Gernot G. Rohde^b ; ^c ; Erik V. Beuken^a ; ^c ; Gert E. Grauls^a ; ^c ; Cathrien A. Bruggeman^a ; ^c ; Frank R. Stassen^a ; ^c ; ^{f.stassen@maastrichtuniversity.nl}
• 关键词：Interferon ¦Â ; Rhinovirus ; A549 cells ; Primary bronchial epithelial cells ; Asthma ; COPD
• 刊名：Journal of Infection
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：66
• 期：2
• 页码：163-169
• 全文大小：483 K

文摘

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Summary

Objectives

Interferon-¦Â (IFN¦Â) induces strong antiviral effects and is therefore an attractive agent to prevent or reduce the incidence of virus-mediated exacerbations in asthmatic or chronic obstructive pulmonary disease (COPD) patients. We therefore investigated the effects of prophylactic IFN¦Â on respiratory epithelial cells infected with rhinovirus (RV).

Methods

A549 cells and primary bronchial epithelial cells (PBECs) were exposed for 18?h to IFN¦Â. Then, IFN¦Â was either removed or maintained in the supernatant for the rest of the experiment and cells were infected with RV-1B at t?=?0 or 72?h after the initial exposure to IFN¦Â.

Results

Viral RNA levels were decreased in both cell types. Furthermore, both viral RNA and infectious virus levels in the supernatant of infected A549 cells were still significantly reduced at 72?h after removal of IFN¦Â. This pronounced antiviral pre-treatment effect was associated with increased expression of the antiviral genes IFN-stimulated protein of MR15000 (ISG15) and?Myxovirus resistance 1 (Mx1) and the effect was maintained even when IFN¦Â levels in the supernatant of A549 cells were undetectable.

Conclusions

These data show that IFN¦Â has not only a strong, but also a long-lasting protective effect against RV infection of respiratory epithelium.