Prevention of lethal respiratory vaccinia infections in mice with interferon-α and interferon-γ

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• 作者：Liu ; Ge ; Zhai ; Qingzhu ; Schaffner ; Dustin J. ; Wu ; Aiguo ; Yohannes ; Adiamseged ; Robinson ; Tanisha M. ; et. al.
• 关键词：Interferon-&#945 ; Interferon-γ ; Antiviral ; Vaccinia virus ; Poxvirus
• 刊名：FEMS Immunology and Medical Microbiology
• 出版年：2004
• 出版时间：April 9, 2004
• 年：2004
• 卷：40
• 期：3
• 页码：201-206
• 全文大小：253 K

文摘

The antiviral efficacy of interferons (IFNs) was evaluated using a vaccinia intranasal infection model in mice in this study. We provide evidence that intranasal administration of IFN-α and IFN-γ (days −1 to +3) resulted in 100 and 90 % survival against a lethal respiratory vaccinia infection (8 LD₅₀) in mice, respectively; whereas no animals in the placebo group survived through the study period (21 days). The IFN treatment consisted of a single daily dose of 5×10³ U per mouse for 5 consecutive days. The efficacy of IFN-γ was evident even when the IFN-γ treatments started 1–2 days after infection and when a lower dose (2×10³ U per mouse) was used. The treatment of IFN-α and IFN-γ reduced the virus titers in the lungs of infected mice by 1000–10 000-fold, when the administration started 1 day after infection. Our data suggest that IFN-α and IFN-γ are effective in protecting vaccinia-infected mice from viral replication in lungs and mortality, and may be beneficial in other human orthopoxvirus infections.