An interlaboratory study on efficient detection of Shiga toxin-producing Escherichia coli O26, O103, O111, O121, O145, and O157 in food using real-time PCR assay and chromogenic agar
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- 作者：Yukiko Hara-Kudoa ; ykudo@nihs.go.jp" class="auth_mail" title="E-mail the corresponding author ; Noriko Konishib ; Kayoko Ohtsukac ; Kaori Iwabuchid ; Rie Kikuchie ; Junko Isobef ; Takumiko Yamazakig ; Fumie Suzukih ; Yuhki Nagaii ; Hiroko Yamadaj ; Atsuko Tanouchik ; Tetsuya Moril ; Hiroshi Nakagawam ; Yasufumi Uedan ; Jun Terajimaa
- 关键词：Shiga toxin-producing Escherichia coli ; O157 ; Non-O157 ; Detection ; Method ; Interlaboratory study
- 刊名：International Journal of Food Microbiology
- 出版年：2016
- 出版时间：2 August 2016
- 年：2016
- 卷：230
- 期：Complete
- 页码：81-88
- 全文大小：873 K
文摘
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We conducted an interlaboratory study on efficient detection for Shiga toxin-producing Escherichia coli.
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Serogroups O26, O103, O111, O121, O145, and O157 were detected in inoculated food.
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The developed real-time PCR assays showed high sensitivity.
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Plating after immunomagnetic separation onto agars showed high sensitivity.
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Efficient, rapid and sensitive detection methods for the six STEC were established.