Monitoring salivary testosterone and cortisol concentrations across an international sports competition: Data comparison using two enzyme immunoassays and two sample preparations

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• 作者：Blair T. Crewther^a ; ^b ; ^{bcrewthe@imperial.ac.uk} ; Emad Al-Dujaili^c ; Nacer F. Smail^c ; Salzitza Anastasova^d ; Liam P. Kilduff^b ; Christian J. Cook^a ; ^b ; ^e ; ^f
• 关键词：Steroids ; Saliva ; Validation ; Athletes ; Elite sport
• 刊名：Clinical Biochemistry
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：46
• 期：4-5
• 页码：354-358
• 全文大小：494 K

文摘

Objectives

Salivary testosterone (T) and cortisol (C) concentrations were monitored across a sports competition. Data were compared using two enzyme-immunoassay (EIA) methods and two sample preparations to determine their influence on hormone concentrations.

Design and methods

A group of male athletes (n = 19) provided a saliva sample the morning before and one day after (24 h post) an international rugby union match. Following an extraction procedure, the samples were analysed for T and C concentrations using a commercial kit (CM^E) and an in-house method (IH^E). Raw samples (no extraction procedure) were also tested using the commercial kit (CM^R).

Results

There were no significant changes in T and C levels from pre to post competition with each EIA method and sample preparation, but significant differences in T (IH^E > CM^E > CM^R) and C (CM^R > IH^E and CM^E) concentrations were seen when both samples were pooled. Bland-Altman analyses confirmed the presence of fixed and proportional bias. Strong and significant correlations were demonstrated between the IH^E and CM^E measures of salivary T (r = 0.93-0.97) and C (r = 0.95-0.97). The T and C values from the raw and extracted samples were also strongly correlated (r = 0.93-0.96).

Conclusions

The measurement of salivary T and C concentrations across an international sports event was influenced by different EIA methods and sample preparations, but all measures were strongly correlated with some bias. Both T and C were unresponsive to the sports event, but within the group results large individual variation was seen.