Reduced faecal shedding of Escherichia coli O157:H7 in cattle following systemic vaccination with γ-intimin C₂₈₀ and EspB proteins

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• 作者：Daniel A. Vilte^a ; Mariano Larzá ; bal^b ; Sergio Garbaccio^a ; Mariela Gammella^a ; Bettina C. Rabinovitz^a ; Ana M. Elizondo^a ; Rodolfo J.C. Cantet^c ; Fernando Delgado^a ; Virginia Meikle^b ; Angel Cataldi^b ; Elsa C. Mercado^a ; ^{emercado@cnia.inta.gov.ar"" rel=""nofollow} ; ^{elsamercado2000@yahoo.com.ar"" rel=""nofollow}
• 关键词：E. coli O157 ; H7 ; Intimin ; EspB ; Vaccine
• 刊名：Vaccine
• 出版年：2011
• 出版时间：23 May 2011
• 年：2011
• 卷：29
• 期：23
• 页码：3962-3968
• 全文大小：365 K

文摘

Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome (HUS) worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-intimin C₂₈₀) and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle.