Characterization of a highly Cu²⁺-selective fluorescent probe derived from rhodamine B

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• 作者：Chunwei Yu ; Ting Wang ; Ke Xu ; Jing Zhao ; Maohua Li ; Shixing Weng ; Jun Zhang
• 关键词：Fluorescent probe ; Fluorescent imaging ; Rhodamine derivative ; Cu2+ ; Fluorescence enhancement ; Living cell
• 刊名：Dyes and Pigments
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：96
• 期：1
• 页码：38-44
• 全文大小：834 K

文摘

A rhodamine B derivative was synthesized and characterized as a highly selective and sensitive probe for Cu²⁺ in ethanol-water solution (2:3, v:v, pH7.4, 50?mM HEPES). A prominent fluorescence enhancement at 575?nm was observed in the presence of Cu²⁺, accompanied by the change in the absorption spectrum. Under the optimal conditions, a good linear range of 0.5-1.5?¦ÌM with a detection limit of 1.6?¡Á?10^?7?M were obtained. Furthermore, confocal laser scanning microscopy experiments have proven that this probe is cell-permeable and can respond to changes in intracellular Cu²⁺ in living cells.