Immobilization of yeast inulinase on chitosan beads for the hydrolysis of inulin in a batch system

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• 作者：R.S. Singh^a ; ^{rssingh11@lycos.com} ; ^{rssbt@pbi.ac.in} ; R.P. Singh^a ; J.F. Kennedy^b
• 关键词：Kluyveromyces marxianus ; Inulinase ; Inulin ; Fructose ; Immobilization ; Chitosan beads
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：87-93
• 全文大小：2143 K
• 卷排序：95

文摘

Extracellular inulinase was partially purified from Kluyveromyces marxianus YS-1. Partially purified enzyme was immobilized on glutaraldehyde activated chitosan beads. Free and immobilized inulinase was used for the hydrolysis of inulin in a batch system. To check the operational stability of the immobilized biocatalyst, recycling of the developed immobilized biocatalyst was carried out for inulin hydrolysis. The developed immobilized enzyme was successfully used for hydrolysis of inulin for 14 batches.