Acoustic Cluster Therapy: In Vitro and Ex Vivo Measurement of Activated Bubble Size Distribution and Temporal Dynamics
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- 作者：Andrew John Healey&lowast ; ; Andrew.healey@phoenixsolutions.no" class="auth_mail" title="E-mail the corresponding author ; Per Christian Sontum&lowast ; ; Svein Kvå ; le&lowast ; ; Morten Eriksen&dagger ; ; Ragnar Bendiksen&Dagger ; ; Audun Tornes§ ; ; Jonny Ø ; stensen¶ ;
- 关键词：Acoustic cluster therapy ; In vitro bubble sizing ; Ex vivo bubble sizing ; In vivo bubble sizing ; Microbubbles ; Droplet vaporization ; Ultrasound contrast agent ; Myocardial perfusion imaging
- 刊名：Ultrasound in Medicine & Biology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：42
- 期：5
- 页码：1145-1166
- 全文大小：1188 K
文摘
Acoustic cluster technology (ACT) is a two-component, microparticle formulation platform being developed for ultrasound-mediated drug delivery. Sonazoid microbubbles, which have a negative surface charge, are mixed with micron-sized perfluoromethylcyclopentane droplets stabilized with a positively charged surface membrane to form microbubble/microdroplet clusters. On exposure to ultrasound, the oil undergoes a phase change to the gaseous state, generating 20- to 40-μm ACT bubbles. An acoustic transmission technique is used to measure absorption and velocity dispersion of the ACT bubbles. An inversion technique computes bubble size population with temporal resolution of seconds. Bubble populations are measured both in vitro and in vivo after activation within the cardiac chambers of a dog model, with catheter-based flow through an extracorporeal measurement flow chamber. Volume-weighted mean diameter in arterial blood after activation in the left ventricle was 22 μm, with no bubbles >44 μm in diameter. After intravenous administration, 24.4% of the oil is activated in the cardiac chambers.