Antifouling activities of anti-histamine compounds against the barnacle Amphibalanus (= Balanus) amphitrite

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• 作者：Cuili Jin ; Jingjing Qiu ; Li Miao ; Ke Feng ; Xiaojian Zhou
• 关键词：Antifouling compounds ; Barnacle Amphibalanus (=  ; Balanus) amphitrite ; Larval settlement and metamorphosis ; Marine biofouling
• 刊名：Journal of Experimental Marine Biology and Ecology
• 出版年：March, 2014
• 年：2014
• 卷：452
• 期：Complete
• 页码：47-53
• 全文大小：314 K

文摘

The barnacle Amphibalanus (= Balanus) amphitrite is a typical marine fouling invertebrate, with adults releasing planktonic larvae that settle and further metamorphose into juveniles on submerged surfaces. The mechanisms regulating larval selection for settlement surface and metamorphosis initiation have been proposed to have a close relationship to neurotransmitters. In this study, a total of 35 signal-influencing pharmaceutical compounds affecting several neurotransmitters were examined in an antifouling bioassay using cyprid larvae of A. amphitrite to search for new antifouling compounds. The results indicated that 18 of the 35 compounds were effective against the larval settlement and metamorphosis of the barnacle A. amphitrite, with nontoxic effects at concentrations of 20 渭g mL^鈭?#xA0;1. By reducing the treatment concentrations, a 5-HT₃ receptor antagonist of Ondansetron; two epinephrine 尾₂ receptor agonists of Propranolol and Clorprenaline; and five histamine H₁ receptor antagonists, including Loratadine, Antazoline, Clemastine, Azelastine, and Triprolidine were found to be very active against the larval settlement and metamorphosis of A. amphitrite, with EC₅₀ values lower than 1.6 渭g mL ^鈭?. In a recovery bioassay, the larvae treated with a histamine H₁ receptor antagonist of Triprolidine regained the ability to settle and to metamorphose into juveniles in fresh artificial seawater. The settlement and metamorphosis of the recovered larvae were further enhanced by the addition of 5 脳 10^鈭?#xA0;9 M histamine. The results of the present study indicated that some of the compounds influencing neurotransmitter signaling, especially histamine H₁ receptor antagonists, are promising new antifouling candidates.