Increasing extracellular H2O2 produces a bi-phasic response in intracellular H2O2, with peroxiredoxin hyperoxidation only triggered once the cellular H2O2-buffering capacity is overwhelmed
详细信息 查看全文
- 作者：Lewis Elwood Tomalina ; Alison Michelle Daya ; Zoe Elizabeth Underwooda ; Graham Robert Smithb ; Piero Dalle Pezzea ; Charalampos Rallisc ; 1 ; Waseema Patela ; Bryan Craig Dickinsond ; Jü ; rg Bä ; hlerc ; Thomas Francis Brewere ; Christopher Joh-Leung Change ; Daryl Pierson Shanleya ; daryl.shanley@ncl.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Elizabeth Ann Veala ; e.a.veal@ncl.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Prx ; peroxiredoxins ; Trx ; thioredoxin ; Txl1 ; thioredoxin-like protein 1 ; Gpx1 ; glutathione peroxidase 1 ; AMS ; 4-acetamido-4ʹ-((iodoacetyl)amino)stilbene-2 ; 2ʹ-disulfonic acid ; NEM ; N-ethylmaleimide ; ROS ; reactive oxygen species ; H2O2 ; hydrogen peroxide ; CysP ; peroxidatic cysteine ; CysR ; resolving cysteine ; Pr-SS ; protein disulfides ; Pr-SH ; protein thiols ; DMEM ; Dulbecco&rsquo ; s Modified Eagles Medium ; EMM ; Edinburgh minimal media ; TCA ; trichloroacetic acid ; DPBS ; Dulbecco&rsquo ; s phosphate-buffer
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：95
- 期：Complete
- 页码：333-348
- 全文大小：4734 K
文摘
- •
Mathematical model simulating in vivo dynamic, H2O2-induced, changes in Prx oxidation.
- •
Increasing extracellular H2O2 produces a bi-phasic response in intracellular H2O2.
- •
Thioredoxin and the thiol-proteome can efficiently buffer low levels of H2O2.
- •
Prx hyperoxidation only occurs once this H2O2 buffering capacity is saturated.