Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN

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• 作者：Dongsheng Guo^a ; ^b ; Haikun Liu^a ; ^b ; Ge Gao^a ; ^b ; Yanli Liu^a ; ^b ; Yuanqi Zhuang^a ; ^b ; Fan Yang^a ; ^b ; ^c ; Kepin Wang^b ; Tiancheng Zhou^b ; Dajiang Qin^b ; Liangqing Hong^e ; Jialiang Li^d ; Kecheng Xu^d ; Yin-xiong Li^a ; ^b ; ^c ; ^d ; ^{li_yinxiong@gibh.ac.cn}
• 刊名：Stem Cell Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：18
• 期：Complete
• 页码：67-69
• 全文大小：433 K
• 卷排序：18

文摘

A patient specific point mutation (c.1288G > T) of Men1 gene was introduced into wide type iPSC line with CRISPR/Cas9 and single-stranded donor oligonucleotides carrying the mutation. The mutated iPSC line has a heterozygous c.1288G > T mutation on exon-9 of Men1 that was confirmed by sequencing analysis. The karyotype of this line was normal and the pluripotency was demonstrated by its ability to differentiate into three germ layers. These artificially created Men1 mutation in wild type iPSC line will help to dissect out the molecular basis of two patients carried the same mutation from one family who were differentially represented hypoglycemia.