- 作者：Ishai Levin ; M.D. ; ishai.levin@gmail.com ; Benny Almog ; M.D. ; Tamar Shwartz ; B.Sc. ; Veronica Gold ; M.Sc. ; Dalit Ben-Yosef ; Ph.D. ; Michal Shaubi ; M.D. ; Ami Amit ; M.D. ; Mira Malcov ; Ph.D.
- 关键词：Polar-body biopsy ; embryo quality ; embryo cleavage ; fragmentation
- 刊名：Fertility and Sterility
- 出版年：2012
- 出版时间：May, 2012
- 年：2012
- 卷：97
- 期：5
- 页码：1085-1088
- 全文大小：79 K
Objective
To evaluate the effect of laser polar-body biopsy (PBB) for preimplantation genetic diagnosis on embryo quality.Study design
Retrospective case-control analysis. The quality of 145 embryos after PBB was compared to 276 embryos of the same group of women without biopsy.
Setting
University-based tertiary-care medical center.
Patient(s)
Women with inherited genetics disease.
Intervention(s)
Laser PBB of IVF embryos for genetic diagnosis.
Main Outcome Measure(s)
The study and control embryos were compared for fertilization rate, pronuclear grading, and cleavage-stage parameters on days 1, 2, and 3 after oocyte retrieval.
Result(s)
The study embryos demonstrated higher rates of cleavage arrest (3.6 % vs. 0.7 % ), higher rate of significant fragmentation on day 2 (9.5 % vs. 3.0 % ), and lower rate of good cleavage embryos on day 2 (69.1 % vs. 78.4 % ) compared with control embryos. On day 3, the study embryos had lower cleavage rates (six or more blastomeres; 56.5 % vs. 74.5 % ), higher fragmentation (11.7 % vs. 3.9 % ), higher rate of embryos presenting inferior cleavage pattern (57.2 % vs. 38.5 % ), and lower mean blastomere number (5.8 ¡À 2.1 vs. 6.6 ¡À 1.9) compared with control embryos.
Conclusion(s)
Polar-body biopsy may have a negative effect on embryo quality.